生理盐水操作步骤
生理盐水操作步骤
生理盐水操作步骤
生理盐水操作步骤

生理盐水操作步骤

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2017-07-20 15:23:13
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上海莼试生物技术有限公司

上海莼试生物技术有限公司

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产品简介

生理盐水操作步骤我司供应的分子生物学试剂,产品齐全,质量保证,*,欢迎广大科研用户选购!

详细介绍

生理盐水操作步骤储存条件:避光、干燥阴凉处封闭贮存,严禁与有毒、有害物品混放、混运。本品为非危险 产品可按一般化学品运输,轻搬动轻放,防止日晒、雨淋!受热、受潮、受光后易丧失活力,保存期短,因此贮存和运输条件比较苛刻。
产品名称:生理盐水操作步骤
英文名字:Normal saline (NS)

产品描述

本产品为渗透压与动物或人体血浆的渗透压相等的溶液,浓度为0.9%。
 
产品优势

为无菌包装。
 
储存条件

4℃保存。
 
注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

生理盐水操作步骤储存条件:
 大部分的试剂产品是比较稳定的,只要您严格按照产品包装或者说明书上的储存条件进行储存,*可以保证其在有效期内的正常使用。一般常见储存条件有零下XX摄氏度,2-8摄氏度,常温等。特别提醒,对于某些酶类制品,如果储存条件是2-8摄氏度,不要储存在零下,否则容易失活,对于某些需要严格避光,防潮,充氮气保护的,接触空气或者过分暴露非常容易氧化或者受潮变质。
低温储存产品,运输途中冷藏问题:
 对于需要低温储存的产品,我们在运输时候都会采用冷藏包装,但是部分由于外界气温过高,运输时间较长,对于普通冷藏包装,途中可能会温度升高,这些都是没有问题的,因为这种普通冷藏只适用于那些短期内(2周)高温依然稳定的产品,建议低温的储存条件,只是长期储存时候的条件,您可以放心使用。对于某些特殊的产品,如抗体,我们会采用干冰冷藏包装,确保全程低温。公司都对产品性状进行了严格的加速稳定测试,行业经验丰富。
生理盐水操作步骤产品应用:
 对于一些相对特殊的产品,我们会提供产品说明书,里面会对产品溶解性,及我们所能了解的产品应用信息,实验方案进行描述,您可以作为参考。对于常见试剂,因为应用广泛,一般不提供说明书。如果您在实验过程中遇到了问题,欢迎与我们讨论,我们将尽力帮您解决。
生理盐水操作步骤分装及溶液配制:
 您收到货物后不要自己进行分包,因为分包环境、包装材料等因素可能导致分包后的产品变质;如您有特殊包装要求,请在订购时候与我们客服代表阐明,当然价格会做适当调整。对于开盖后,长期未使用的,请务必重新密封好,建议Parafilm封口膜,并按照相应储存条件使用。如果放置时间过长,超过产品有效期,建议您重新购买,以免影响实验质量。
10ml    瓶    His标签纯化树脂(50%浆料)
5ml    瓶    GST标签纯化树脂(50%浆料)
10ml    瓶    GST标签纯化树脂(50%浆料)
        蛋白质定量
5g    瓶    Albumin BovineⅤ 牛血清白蛋白Ⅴ
10g    瓶    Albumin BovineⅤ 牛血清白蛋白Ⅴ
25g    瓶    Albumin BovineⅤ 牛血清白蛋白Ⅴ
50g    瓶    Albumin BovineⅤ 牛血清白蛋白Ⅴ
100g    瓶    Albumin BovineⅤ 牛血清白蛋白Ⅴ
50ml    瓶    Folin-Phenol 福林酚 (进口)
50ml    瓶    Folin-Phenol 福林酚
1ml    支    BSA标准品5mg-ml
100T    盒    双缩脲法蛋白含量测定试剂盒
2500微孔    盒    Bradford蛋白浓度测定试剂盒(250T)
100ml    瓶    5×考马斯亮蓝G-250(蛋白定量用)
500ml    瓶    5×考马斯亮蓝G-250(蛋白定量用)
500微孔    盒    BCA蛋白浓度测定试剂盒(500T)
1g    瓶    2,2-联喹啉-4,4-二甲酸二钠 BCA
1000微孔    盒    Lowry法蛋白浓度测定试剂盒(100T)
        蛋白质电泳
100ml    瓶    30%丙烯酰胺(29:1)
500ml    瓶    30%丙烯酰胺(29:1)
100ml    瓶    40%丙烯酰胺(37.5:1)
500ml    瓶    40%丙烯酰胺(37.5:1)

中文名称  亲环蛋白(亲环素)PPIA抗体 
别    名  Cyclophilin A; CyclophilinA; Cyclosporin A binding protein; Cyclosporin A-binding protein; CYPA; CYPH; Peptidyl prolyl cis trans isomerase A; Peptidyl-prolyl cis-trans isomerase A; Peptidylprolyl isomerase A; PPIA; PPIA protein; PPIA_HUMAN; PPIase A; Rotamase A; RotamaseA; T cell cyclophilin.  
浓    度  1mg/1ml 
规 格  0.1ml/100μg  0.2ml/200μg 
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Rabbit
产品类型  一抗    
研究领域  免疫学 信号转导 t-淋巴细胞  
蛋白分子量  predicted molecular weight: 18kDa 
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthetic peptide derived from human Cyclophilin A. 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 
产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 
(石蜡切片需做抗原修复) 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity (1). Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins (2,3). Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans (4,5). CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain (6). CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication (7,8). Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes (9,10).
Function : PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Subcellular Location : Cytoplasm.
Similarity : Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain.
英文名称  Anti-PTN/Pleiotrophin 
中文名称  多效生长因子抗体 
别    名  pleiotrophin; HARP; HBBM; HBGAM; HBGF8; HBNF; HBNF1; Heparin Binding Growth Factor 8; Heparin binding neurite outgrowth promoting factor 1; heparin-binding growth associated molecule; NEGF1; Neurite Growth Promoting Factor 1; OSF-1; osteoblast-specific factor; PTN; PTN_HUMAN. 
浓    度  1mg/1ml 
规 格  0.1ml/100μg  0.2ml/200μg 
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  Human, Mouse, Rat, Pig, Cow, Rabbit
产品类型  一抗    
研究领域  肿瘤 细胞生物 神经生物学 信号转导 生长因子和激素 细胞骨架 细胞外基质  
蛋白分子量  predicted molecular weight: 15kDa 
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthetic peptide derived from human PTN C-terminus 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 
产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 
(石蜡切片需做抗原修复) 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 Pleiotrophin is a heparin-binding growth factor involved in the differentiation and proliferation of neuronal tissue during embryogenesis, and also secreted by melanoma and breast carcinoma cells. Pleiotrophin exhibits mitogenic and angiogenic properties and has been shown to influence the vascular supply, expansion and metastasis of tumour cells.
Function : Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation.
Subunit : Interacts with ALK and NEK6.
Subcellular Location : Secreted.
Tissue Specificity : Osteoblast and brain.
Post-translational modifications : Phosphorylated by NEK6.
Similarity : Belongs to the pleiotrophin family.
Database links : UniProtKB/Swiss-Prot: P21246.1
生理盐水操作步骤多效生长因子(pleiotrophin)是一种分泌性的生长因子,具有促进肿瘤细胞的增殖与迁移,对肿瘤血管的生成有促进作用。
英文名称  Anti-PCDHA2 
中文名称  原钙粘附蛋白α2抗体 
别    名  KIAA0345 like 12; PCDH alpha 2; PCDH ALPHA2; Protocadherin alpha 2; PCDA2_HUMAN. 

 

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