转录调控-DAP-seq

100+物种，2000+转录因子的DAP-seq实战经验，无需抗体，高通量鉴定转录因子的下游基因，蓝景科信已助力客户在许多期刊发表文章，例如：Molecular Plant，The Plant Cell，Plant Physiology，Plant Biotechnology Journal，Journal of Integrative Plant Biology，New Phytologist等。

在功能基因组学和表观遗传学研究中，转录因子结合位点（TFBS）的发掘一直是研究热点。传统的ChIP-seq（染色质免疫共沉淀测序）方法，在抗体质量很好的情况下能够有效检测到TFBS。然而，好的抗体可遇不可求，这限制了ChIP-seq更广泛的应用。

DAP-seq技术的出现，使TFBS 的研究不再局限于物种，不再受抗体质量的限制，为生命科学领域转录因子的研究提供了新的有效工具。

合作案例：

Yao J, Shen Z, Zhang Y, Wu X, Wang J, Sa G, Zhang Y, Zhang H, Deng C, Liu J, Hou S, Zhang Y, Zhang Y, Zhao N, Deng S, Lin S, Zhao R, Chen S. Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance. J Exp Bot. 2020 Feb 19;71(4):1527-1539. doi: 10.1093/jxb/erz493. PMID: 31680166; PMCID: PMC7031066.

Tang N, Cao Z, Yang C, Ran D, Wu P, Gao H, He N, Liu G, Chen Z. A R2R3-MYB transcriptional activator LmMYB15 regulates chlorogenic acid biosynthesis and phenylpropanoid metabolism in Lonicera macranthoides. Plant Sci. 2021 Jul;308:110924. doi: 10.1016/j.plantsci.2021.110924. Epub 2021 Apr 29. PMID: 34034872.

Yu Z, Chen X, Li Y, Shah SHA, Xiao D, Wang J, Hou X, Liu T, Li Y. ETHYLENE RESPONSE FACTOR 070 inhibits flowering in Pak-choi by indirectly impairing BcLEAFY expression. Plant Physiol. 2024 Jan 25:kiae021. doi: 10.1093/plphys/kiae021. Epub ahead of print. PMID: 38269601.

Cao X, Li X, Su Y, Zhang C, Wei C, Chen K, Grierson D, Zhang B. Transcription factor PpNAC1 and DNA demethylase PpDML1 synergistically regulate peach fruit ripening. Plant Physiol. 2023 Nov 22:kiad627. doi: 10.1093/plphys/kiad627. Epub ahead of print. PMID: 37992120.

Zhao H, Wan S, Huang Y, Li X, Jiao T, Zhang Z, Ma B, Zhu L, Ma F, Li M. The transcription factor MdBPC2 alters apple growth and promotes dwarfing by regulating auxin biosynthesis. Plant Cell. 2024 Feb 26;36(3):585-604. doi: 10.1093/plcell/koad297. PMID: 38019898; PMCID: PMC10896295.

Meiran Li1, Mingwei Chen, Yongli Zhang, Longgang Zhao, Jiancheng Zhang, Hui Song, Identification of the target genes of AhTWRKY24 and AhTWRKY106 transcription factors reveals their regulatory network in Arachis hypogaea cv. Tifrunner using DAP-seq[J].Oil Crop Science.2023,Vol.8(No.2)：89-96.

Liu P, Bu C, Chen P, El-Kassaby YA, Zhang D, Song Y. Enhanced genome-wide association reveals the role of YABBY11-NGATHA-LIKE1 in leaf serration development of Populus. Plant Physiol. 2023 Mar 17;191(3):1702-1718. doi: 10.1093/plphys/kiac585. PMID: 36535002; PMCID: PMC10022644.

Yuan J, Liu X, Zhao H, Wang Y, Wei X, Wang P, Zhan J, Liu L, Li F, Ge X. GhRCD1 regulates cotton somatic embryogenesis by modulating the GhMYC3-GhMYB44-GhLBD18 transcriptional cascade. New Phytol. 2023 Oct;240(1):207-223. doi: 10.1111/nph.19120. Epub 2023 Jul 11. PMID: 37434324.

Dong, Q., Tian, Y., Zhang, X., Duan, D., Zhang, H., Yang, K., Jia, P., Luan, H., Guo, S., Qi, G., Mao, K., & Ma, F. (2023). Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple. Horticultural Plant Journal.

Fang Y, Wang D, Xiao L, Quan M, Qi W, Song F, Zhou J, Liu X, Qin S, Du Q, Liu Q, El-Kassaby YA, Zhang D. Allelic variation in transcription factor PtoWRKY68 contributes to drought tolerance in Populus. Plant Physiol. 2023 Aug 31;193(1):736-755. doi: 10.1093/plphys/kiad315. PMID: 37247391; PMCID: PMC10469405.

Zhu J, Wei X, Yin C, Zhou H, Yan J, He W, Yan J, Li H. ZmEREB57 regulates OPDA synthesis and enhances salt stress tolerance through two distinct signalling pathways in Zea mays. Plant Cell Environ. 2023 Sep;46(9):2867-2883. doi: 10.1111/pce.14644. Epub 2023 Jun 16. PMID: 37326336.

Han P, Hua Z, Zhao Y, Huang L, Yuan Y. PuCRZ1, an C2H2 transcription factor from Polyporus umbellatus, positively regulates mycelium response to osmotic stress. Front Microbiol. 2023 Apr 6;14:1131605. doi: 10.3389/fmicb.2023.1131605. PMID: 37089566; PMCID: PMC10115967.

Zhang S, Wang L, Yao J, Wu N, Ahmad B, van Nocker S, Wu J, Abudureheman R, Li Z, Wang X. Control of ovule development in Vitis vinifera by VvMADS28 and interacting genes. Hortic Res. 2023 Apr 13;10(6):uhad070. doi: 10.1093/hr/uhad070. PMID: 37293531; PMCID: PMC10244803.

Wang L, Tian T, Liang J, Li R, Xin X, Qi Y, Zhou Y, Fan Q, Ning G, Becana M, Duanmu D. A transcription factor of the NAC family regulates nitrate-induced legume nodule senescence. New Phytol. 2023 Jun;238(5):2113-2129. doi: 10.1111/nph.18896. Epub 2023 Apr 9. PMID: 36945893.

Li M, Hou L, Zhang C, Yang W, Liu X, Zhao H, Pang X, Li Y. Genome-Wide Identification of Direct Targets of ZjVND7 Reveals the Putative Roles of Whole-Genome Duplication in Sour Jujube in Regulating Xylem Vessel Differentiation and Drought Tolerance. Front Plant Sci. 2022 Feb 4;13:829765. doi: 10.3389/fpls.2022.829765. PMID: 35185994; PMCID: PMC8854171.

Yang Z, Liu Z, Ge X, Lu L, Qin W, Qanmber G, Liu L, Wang Z, Li F. Brassinosteroids regulate cotton fiber elongation by modulating very-long-chain fatty acid biosynthesis. Plant Cell. 2023 May 29;35(6):2114-2131. doi: 10.1093/plcell/koad060. PMID: 36861340; PMCID: PMC10226586.

Sun Y, Han Y, Sheng K, Yang P, Cao Y, Li H, Zhu QH, Chen J, Zhu S, Zhao T. Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of pigment glands in Gossypium bickii. Mol Plant. 2023 Apr 3;16(4):694-708. doi: 10.1016/j.molp.2023.02.005. Epub 2023 Feb 10. PMID: 36772793.

Liu Y, Liu Q, Li X, Zhang Z, Ai S, Liu C, Ma F, Li C. MdERF114 enhances the resistance of apple roots to Fusarium solani by regulating the transcription of MdPRX63. Plant Physiol. 2023 Jul 3;192(3):2015-2029. doi: 10.1093/plphys/kiad057. PMID: 36721923; PMCID: PMC10315273.

Liu YN, Wu FY, Tian RY, Shi YX, Xu ZQ, Liu JY, Huang J, Xue FF, Liu BY, Liu GQ. The bHLH-zip transcription factor SREBP regulates triterpenoid and lipid metabolisms in the medicinal fungus Ganoderma lingzhi. Commun Biol. 2023 Jan 3;6(1):1. doi: 10.1038/s42003-022-04154-6. PMID: 36596887; PMCID: PMC9810662.

Liu L, Chen G, Li S, Gu Y, Lu L, Qanmber G, Mendu V, Liu Z, Li F, Yang Z. A brassinosteroid transcriptional regulatory network participates in regulating fiber elongation in cotton. Plant Physiol. 2023 Mar 17;191(3):1985-2000. doi: 10.1093/plphys/kiac590. PMID: 36542688; PMCID: PMC10022633.

Bi Y, Wang H, Yuan X, Yan Y, Li D, Song F. The NAC transcription factor ONAC083 negatively regulates rice immunity against Magnaporthe oryzae by directly activating transcription of the RING-H2 gene OsRFPH2-6. J Integr Plant Biol. 2023 Mar;65(3):854-875. doi: 10.1111/jipb.13399. Epub 2023 Feb 23. PMID: 36308720.

Guo X, Yu X, Xu Z, Zhao P, Zou L, Li W, Geng M, Zhang P, Peng M, Ruan M. CC-type glutaredoxin, MeGRXC3, associates with catalases and negatively regulates drought tolerance in cassava (Manihot esculenta Crantz). Plant Biotechnol J. 2022 Dec;20(12):2389-2405. doi: 10.1111/pbi.13920. Epub 2022 Sep 14. PMID: 36053917; PMCID: PMC9674314.

Tang N, Cao Z, Yang C, Ran D, Wu P, Gao H, He N, Liu G, Chen Z. A R2R3-MYB transcriptional activator LmMYB15 regulates chlorogenic acid biosynthesis and phenylpropanoid metabolism in Lonicera macranthoides. Plant Sci. 2021 Jul;308:110924. doi: 10.1016/j.plantsci.2021.110924. Epub 2021 Apr 29. PMID: 34034872.

Chai Z, Fang J, Huang C, Huang R, Tan X, Chen B, Yao W, Zhang M. A novel transcription factor, ScAIL1, modulates plant defense responses by targeting DELLA and regulating gibberellin and jasmonic acid signaling in sugarcane. J Exp Bot. 2022 Nov 2;73(19):6727-6743. doi: 10.1093/jxb/erac339. PMID: 35986920.

Li R, Zheng W, Yang R, Hu Q, Ma L, Zhang H. OsSGT1 promotes melatonin-ameliorated seed tolerance to chromium stress by affecting the OsABI5-OsAPX1 transcriptional module in rice. Plant J. 2022 Oct;112(1):151-171. doi: 10.1111/tpj.15937. Epub 2022 Aug 19. PMID: 35942609.

Li Q, Zhou L, Chen Y, Xiao N, Zhang D, Zhang M, Wang W, Zhang C, Zhang A, Li H, Chen J, Gao Y. Phytochrome interacting factor regulates stomatal aperture by coordinating red light and abscisic acid. Plant Cell. 2022 Oct 27;34(11):4293-4312. doi: 10.1093/plcell/koac244. PMID: 35929789; PMCID: PMC9614506.

Luo M, Lu B, Shi Y, Zhao Y, Wei Z, Zhang C, Wang Y, Liu H, Shi Y, Yang J, Song W, Lu X, Fan Y, Xu L, Wang R, Zhao J. A newly characterized allele of ZmR1 increases anthocyanin content in whole maize plant and the regulation mechanism of different ZmR1 alleles. Theor Appl Genet. 2022 Sep;135(9):3039-3055. doi: 10.1007/s00122-022-04166-0. Epub 2022 Jul 5. PMID: 35788748.

Wei H, Xu H, Su C, Wang X, Wang L. Rice CIRCADIAN CLOCK ASSOCIATED 1 transcriptionally regulates ABA signaling to confer multiple abiotic stress tolerance. Plant Physiol. 2022 Sep 28;190(2):1057-1073. doi: 10.1093/plphys/kiac196. PMID: 35512208; PMCID: PMC9516778.

相关服务：

1、 凝胶阻滞实验（EMSA）：DAP-seq后续验证服务。

2、 酵母单杂交：DAP-seq后续验证服务

3、 ChIP-seq：高效检测重组蛋白、转录因子在基因组的结合位点

4、 DAP-seq与RNA-seq联合分析： 分析转录因子的靶基因在RNA-seq数据中的表达变化，深入挖掘DAP-seq和RNA-seq测序数据，增加转录组测序的分析深度。

5、 DNA-pull down：鉴定与DNA结合的蛋白。

6、 精美的论文图片设计与制作：专业设计师，设计精美论文插图，提升论文的严谨性和美观度。

转录调控-DAP-seq

DAP-seq是基于DNA亲和纯化，通过体外表达转录因子鉴定TFBS的技术，具有不受抗体和物种限制，且高通量的优势，自该技术问世以来，已被广泛应用于转录调控和表观组学的研究。能帮助您快速找到转录因子的结合位点，寻找转录因子调控的靶基因。

蓝景科信河北生物科技有限公司是一家专业从事生命科学产品和技术开发的公司。本公司拥有雄厚的技术实力，创始团队均具有博士学位，来自于国内的清华大学、中国农业大学和美国麻省理工学院。公司立足保定，放眼中外，以严谨的科学态度，不断满足生命科学领域的市场需求。