BERGAMO-Thorlabs Bergamo® II系列多光子显微镜-森泉（上海）光电科技有限公司手机版

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Thorlabs Bergamo® II系列多光子显微镜

遵循显微镜设计 bi 须符合样品要求的原则，创建了一套 wan 全模块化的成像平台，可以满足多种实验需求。与传统的研究技术(例如使用微电极)相比，我们的多光子成像系统能够以更快速度、更大深度和更高强度同时读出和操纵神经元群。如下面的选项概览部分和右侧较下方的旋转Bergamo显微镜的五轴运动视频所示，我们开发了一个旋转主体，使显微镜可绕样品旋转。我们还提供各种刚性主体，其具有业界 ling,先的7.74英寸高度，可围绕物镜提供较大的三维工作空间。

Thorlabs Bergamo® II系列多光子显微镜

亮点

● 基于贝塞尔光束的快速体积成像

● 三光子成像

● 空间光调制器用于同时多点刺激

Thorlabs Bergamo® II系列多光子显微镜

基于贝塞尔光束的快速体积成像

Thorlabs与Howard Hughes yi,学院以及Na Ji教授(加利福尼亚大学伯克利分校)合作，为我们的Bergamo®多光子激光扫描显微镜提供了贝塞尔光束模块。神经元活动的体内体积成像需要亚微米级空间分辨率和毫秒级时间分辨率。传统方法通过连续扫描衍射 ji 限高斯光束以创建3D图像，而基于贝塞尔光束的多光子成像依靠轴向延伸的焦点来捕获体积图像。激发光束的扩展景深可创建3D体积的2D投影，从而 you 效地将2D帧速率转换为3D体积速率。

Thorlabs Bergamo® II系列多光子显微镜

单次贝塞尔扫描(左)可捕获与堆叠45个光学切片(右)而创建的高斯体积扫描相同的结构信息，从而将总扫描时间减少45倍。图像显示了300 μm x 300 μm范围内扫描的大脑切片。高斯堆叠的扫描深度由比例尺指示。样本由加州大学伯克利分校物理系Ji实验室的Qinrong Zhang博士和Matthew Jacobs提供。

规格

Laser Scanning
Scan Path Wavelength Range		450 - 1100 nm, 680 - 1300 nm, or 800 - 1800 nm
Scan Paths		Resonant-Galvo-Galvo Scanner, Galvo-Resonant Scanners, Galvo-Galvo Scanners, or Spatial Light Modulator; Single or Dual Scan Paths
Scan Speed	8 kHz Resonant-Galvo-Galvo or Galvo-Resonant	2 fps at 4096 x 4096 Pixels 30 fps at 512 x 512 Pixels 400 fps at 512 x 32 Pixels
	12 kHz Resonant-Galvo-Galvo or Galvo-Resonant	4.4 fps at 2048 x 2048 Pixels 45 fps at 512 x 512 Pixels 600 fps at 512 x 32 Pixels
	Galvo-Galvo	3 fps at 512 x 512 Pixels 48 fps at 512 x 32 Pixels 70 fps at 32 x 32 Pixels Pixel Dwell Time: 0.4 to 20 µs
Galvo-Galvo Scan Modes		Imaging: Line, Polyline, Square, or Rectangle Non-Imaging: Circle, Ellipse, Polygon, or Point
Field of View		FN40 Equivalent to 40 mm Diagonal Square (Max) at the Intermediate Image Plane >2.8 mm x 2.8 mm at the Sample plane with a 10X Objective FN20 Equivalent to 20 mm Diagonal Square (Max) at the Intermediate Image Plane >1.4 mm x 1.4 mm at the Sample plane with a 10X Objective
Scan Zoom		1X to 16X (Continuously Variable)
Scan Resolution		Up to 2048 x 2048 Pixels (Bi-Directional) [Up to 1168 x 1168 Pixels for 12 kHz Scanners] Up to 4096 x 4096 Pixels (Unidirectional) [Up to 2336 x 2336 Pixels for 12 kHz Scanners]
Compatible Objective Threadings		M34 x 1.0, M32 x 0.75, M25 x 0.75, and RMS

Spatial Light Modulator (SLM)
Stimulation Area (with a 16X Objective)	600 µm x 600 µm (X and Y) ±150 µm (Z)
Resolution	1024 pixels x 1024 pixels
Maximum Pattern Refresh Rate	4 ms

Multiphoton Signal Detection
Epi Detection	Up to Four Ultrasensitive GaAsP PMTs, Cooled or Non-Cooled
Forward-Direction Detection	Two Ultrasensitive GaAsP PMTs
Maximum of Four PMTs Controlled by the Software at a Given Time
Collection Optics	8°, 10°, or 14° Collection Angle (Angles Quoted When Using an Objective with a 20 mm Entrance Pupil) Easy-to-Exchange Emission Filters and Dichroic Mirrors

Confocal Imaging
Motorized Pinhole Wheel with 16 Round Pinholes from Ø25 µm to Ø2 mm Two to Four Laser Lines (488 nm Standard; Other Options Range from 405 nm to 660 nm) Standard Multialkali or High-Sensitivity GaAsP PMTs Easy-to-Exchange Emission Filters and Dichroic Mirrors

Widefield Viewing
Manual or Motorized Switching Between Scanning and Widefield Modes Illumination Provided via LED or Liquid Light Guide C-Mount Threads for Scientific Cameras

Transmitted Light Imaging
Differential Interference Contrast (DIC) or Dodt Gradient Contrast Widefield or Laser Scanned Illumination Provided by Visible and/or NIR LEDs Compatible with Air or Oil Immersion Condensers

Three-Photon Imaging
Scan Optics for 800 - 1800 nm Range Achieve Reduced Background Scatter for Greater Sensitivity in Deep-Tissue Imaging

Volume Imaging Using Bessel Beams
3D Volumetric Functional Imaging at Video Frame Rates Enhanced Temporal Resolution for Studying Internal Systems at Cellular Lateral Resolution In Vivo

Translation
Microscope Body Rotation (Rotating Bodies Only)	0° to 90° or -45° to +45° Around Objective Focus 0.1° Encoder Resolution
Coarse Elevator Base Z (Rotating Bodies Only)	5" (127 mm) Total Travel; 1 µm Encoder Resolution
Fine Microscope Body X and Y	2" (50.8 mm) Total Travel; 0.5 µm Encoder Resolution
Fine Microscope Arm Z	1" (25.4 mm) Total Travel; 0.1 µm Encoder Resolution

Fine Z Focus
Piezo Objective Scanner	Open Loop: 600 µm ± 10% Travel Range; 1 nm Resolution Closed Loop: 450 µm Travel Range; 3 nm Resolution
Vibrationless Remote Focus	16 Discrete Steps ~400 µm Travel Range with a 10X Objective ~160 µm Travel Range with a 16X Objective

Thorlabs Bergamo® II系列多光子显微镜

森泉为您的科研事业添砖加瓦：

激光控制：激光电流源、激光器温控器、激光器控制、伺服设备与系统等等

探测器：光电探测器、单光子计数器、单光子探测器、CCD、光谱分析系统等等

定位与加工：纳米定位系统、微纳运动系统、多维位移台、旋转台、微型操作器等等

光源：半导体激光器、固体激光器、单频激光器、单纵模激光器、窄线宽激光器、光通讯波段激光器、CO2激光器、中红外激光器、染料激光器、飞秒超快激光器等等

光机械件：用于光路系统搭建的 gao 品 zhi 无应力光机械件，如光学调整架、镜架、支撑杆、固定底座等等

光学平台：主动隔振平台、气浮隔振台、实验桌、刚性工作台、面包板、隔振、隔磁、隔声综合解决方案等等

光学y件：各类晶体、光纤、偏转镜、反射镜、tou射镜、半透半反镜、滤光片、衰减片、玻片等等

染料：激光染料、荧光染料、光致变色染料、光致发光染料、吸收染料等等

产品简介

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