Anti-EMR1/FITC抗体使用说明书
时间:2014-11-25 阅读:540
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时间:2014-11-25 阅读:540
提供商
上海博研生物工程研究中心资料大小
31744资料图片
下载次数
159次资料类型
doc浏览次数
540次Anti-EMR1/FITC抗体使用说明书
Catalog Number : BYK-7058R-FITC
Quantity size : 100ug (2mg/1ml, Lyophilized or Liquid. Buffer = 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide. Reconstitute with 50ul sterile distilled water.)
Background: The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1–3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1–3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages.
Also known as: F4/80; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1; EMR-1; EMR1 hormone receptor; EMR1_HUMAN; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3.
Specificity: -Rabbit Polyclonal IgG, affinity purified by Protein A.
-FITC conjugated
-Reacts with: Human, Mouse, Rat, Pig, Guinea Pig
-Immunogen: KLH conjugated synthetic peptide derived from human EMR1/Gpf480.
-Predicted molecular weight: 95kDa
Application: Flow-Cyt=1:50-200 IF=1:50-200
ICC=1:50-200
Excitation spectrum: 490-495nm
Emission spectrum: 520-530nm
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw
cycles. The Lyophilized or Liquid antibody is stable at room
temperature for at least one month and for greater than a year when
kept at -20 °C. When reconstituted in sterile pH 7.4 0.01M PBS or
diluent of antibody the antibody is stable for at least two weeks at
2-4 °C.
Important Note: This product as supplied is intended for research
use only, not for use in human, therapeutic or diagnostic
applications..