沪鼎产品文献:人IL-6,TNF-α,iNOS ELISA试剂盒引用文献

【文献标题】Rapeseed protein-derived ACE inhibitory peptides LY, RALP and GHS show

antioxidant and anti-inflammatory effects on spontaneously hypertensive rats

【作者】Rong He，Yujiao Wang，Yijie Yang，et.al

【作者单位】南京财经大学（Nanjing University of Finance and Economics）

【文献中引用产品】

人白细胞介素6(IL-6)ELISA试剂盒

人肿瘤坏死因子α(TNF-α)ELISA试剂盒

人诱导型一氧化氮合成酶(iNOS)ELISA试剂盒

【关键词】Rapeseed peptide，ACE inhibition，Anti-inflammatory effect，Cytokine，Oxidative  stress

【DOI】doi.org/10.1016/j.jff.2019.02.031

【影响因子(IF)】5.9

【出版期刊】《Journal of Functional Foods》

【产品原文引用】

Materials and reagents

RAW264.7 cells were obtained from the cell bank of the Chinese Academy of Sciences(Shanghai,China). LPS from Escherichia coli O55:B5,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT), fetal bovine serum, and hematoxylin and eosin (H&E) solutions were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai,China). The SOD Assay Kit, Malondialdehyde (MDA) Assay Kit, Nitric Oxide (NO) Assay Kit and Nitric Oxide Synthase (NOS) Assay Kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China).The Human Interleukin 6 (IL-6) ELISA Kit, Tumor Necrosis Factor-α(TNF-α) ELISA Kit and Inducible Nitric Oxide Synthase (iNOS) Assay Kit were purchased from Shanghai HuDing Biological Technology Co., Ltd.(Shanghai, China). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China).All other chemical reagents were of analytical grade. The ACE inhibitory peptides Leu-Tyr (LY), Arg-Ala-Leu-Pro (RALP) and Gly-His-Ser(GHS) were synthesized with purity over 95% by Synthgene Biological Technology Co., Ltd. (Shanghai, China).

沪鼎产品文献:人Tau蛋白(TAU)ELISA试剂盒引用文献

【文献标题】Effect of erythropoietin combined with hypothermia on serum tau protein levels and neurodevelopmental outcome in neonates with hypoxic-ischemic encephalopathy

【作者】Hong-yan Lv，Su-jing Wu，Qiu-li Wang，et.al

【作者单位】邯郸市邯郸妇幼保健院（Handan Maternal and Child Health Care Hospital, Handan）

【文献中引用产品】

人Tau蛋白(TAU)ELISA试剂盒

【关键词】nerve regeneration; erythropoietin; hypothermia; hypoxic-ischemic encephalopathy; neonate; tau protein; biomarkers; prognosis; neuroprotection; neural regeneration

【DOI】doi.org/10.4103/1673-5374.217338

【影响因子(IF)】3.6

【出版期刊】《NEURAL REGENERATION RESEARCH》

【产品原文引用】

Serum tau protein measurement

Blood samples were taken from the radial vein at 24 hours after birth (before EPO treatment) and 4, 8, and 12 days after EPO treatment. Blood samples were placed in a test tube (specifications: 5 mL, No A06277602; Hebi City Zhong Xing Medical Supplies, Hebi, China), maintained without stirring for 30 minutes at room temperature, and then centrifuged in a L-400 low-speed automatic balancing centrifuge (Hunan Xiangyi Laboratory Instrument Development, Changsha, Hunan Province, China) at 3,000 r/min for 15 minutes.Serum was removed into another test tube and placed in a freezer at ?70°C until use. To avoid any influence on the results, blood transfusions to the neonates were avoided before collecting blood samples. Serum tau protein was detected using an enzyme-linked immunosorbent assay. The kit was provided by the Shanghai HuDing Biological Science and Technology Co., Ltd.

沪鼎产品文献:人白介素4,6,8,10,HGF,TIMP-1等ELISA试剂盒引用文献

【文献标题】水凝胶三维培养对人羊膜间充质干细胞特性及旁分泌效应的影响

【作者】王 旗，杨晓双，王达利

【作者单位】遵义医科大学

【文献中引用产品】

人白细胞介素4(IL-4)ELISA试剂盒

人白细胞介素6(IL-6)ELISA试剂盒

人白细胞介素8(IL-8)ELISA试剂盒

人白细胞介素10(IL-10)ELISA试剂盒

人肝细胞生长因子(HGF)ELISA试剂盒

人金属蛋白酶组织抑制因子-1(TIMP-1)ELISA试剂盒

人基质金属蛋白酶1(MMP-1)ELISA检测试剂盒

人表皮生长因子（EGF）ELISA试剂盒

人转化生长因子β1(TGF-β1)ELISA试剂盒

人碱性成纤维细胞生长因子(bFGF)ELISA试剂盒

人角化细胞生长因子(KGF)ELISA试剂盒

人血管内皮生长因子(VEGF)ELISA试剂盒

【关键词】水凝胶；羊膜间充质干细胞；二维培养；三维培养；创面修复；旁分泌效应

【DOI】10.3969/j.issn.2095-4344.2299 

【影响因子】1.1

【出版期刊】《中国组织工程研究》

【产品原文引用】

ELISA法检测 :

ELISA法检测两种培养条件下hAMSCs旁分泌创面愈合相关因子的蛋白表达情况。将hAMSCs按照2×104

/cm2接种于两种不同培养条件的24孔板上。根据二维培养的细胞情况，细胞贴壁后用PBS清洗两种培养条件下的hAMSCs并更换新鲜培养基，放入细胞培养箱继续培养48 h。48 h后收集两种细胞培养液， 1 000×g离心20 min，取上清。根据Elisa试剂盒(上海沪鼎)说明书进行加样，在450 nm波长处测定各孔的吸光度值。绘制标准曲线，计算白细胞介素4、白细胞介素6、白细胞介素8、白细胞介素10、肝细胞生长因子、金属蛋白酶组织抑制因子、基质金属蛋白酶、表皮生长因子、转化生长因子β1、碱性成纤维细胞因子、角化细胞生长因子、血管内皮生长因子的相应浓度。

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