【文献标题】Rapeseed protein-derived ACE inhibitory peptides LY, RALP and GHS show

antioxidant and anti-inflammatory effects on spontaneously hypertensive rats
【作者】Rong He，Yujiao Wang，Yijie Yang，et.al
【作者单位】南京财经大学（Nanjing University of Finance and Economics）
【文献中引用产品】
人白细胞介素6(IL-6)ELISA试剂盒
人肿瘤坏死因子α(TNF-α)ELISA试剂盒
人诱导型一氧化氮合成酶(iNOS)ELISA试剂盒
【关键词】Rapeseed peptide，ACE inhibition，Anti-inflammatory effect，Cytokine，Oxidative  stress
【DOI】doi.org/10.1016/j.jff.2019.02.031
【影响因子(IF)】3.78
【出版期刊】《Journal of Functional Foods》
【产品原文引用】
Materials and reagents
RAW264.7 cells were obtained from the cell bank of the Chinese Academy of Sciences(Shanghai,China). LPS from Escherichia coli O55:B5,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT), fetal bovine serum, and hematoxylin and eosin (H&E) solutions were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai,China). The SOD Assay Kit, Malondialdehyde (MDA) Assay Kit, Nitric Oxide (NO) Assay Kit and Nitric Oxide Synthase (NOS) Assay Kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China).The Human Interleukin 6 (IL-6) ELISA Kit, Tumor Necrosis Factor-α(TNF-α) ELISA Kit and Inducible Nitric Oxide Synthase (iNOS) Assay Kit were purchased from Shanghai HuDing Biological Technology Co., Ltd.(Shanghai, China). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China).All other chemical reagents were of analytical grade. The ACE inhibitory peptides Leu-Tyr (LY), Arg-Ala-Leu-Pro (RALP) and Gly-His-Ser(GHS) were synthesized with purity over 95% by Synthgene Biological Technology Co., Ltd. (Shanghai, China).

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