沪鼎产品文献:植物MgCh,Rubisco,ELISA试剂盒引用文献
时间:2020-09-30 阅读:534
-
提供商
上海沪鼎生物科技有限公司 -
资料大小
1190546 -
资料图片
-
下载次数
64次 -
资料类型
pdf -
浏览次数
534次
时间:2020-09-30 阅读:534
提供商
上海沪鼎生物科技有限公司资料大小
1190546资料图片
下载次数
64次资料类型
pdf浏览次数
534次【文献标题】Transcriptome profiling of genes involved in photosynthesis in Elaeagnus angustifolia L. under salt stress
【作者】J. LIN, J.P. LI, F. YUAN,et.al
【作者单位】山东师范大学(Shandong Normal University)
【文献中引用产品】
植物镁螯合酶(MgCh) ELISA试剂盒
植物Rubisco活化酶(RCA)ELISA试剂盒
【关键词】biomass; greening; ion concentrations; photosynthetic parameters; plant height.
【DOI】doi.org/10.1007/s11099-018-0824-6
【影响因子(IF)】2.3
【出版期刊】《PHOTOSYNTHETICA》
【产品原文引用】
Protein content and activity of magnesium chelatase and Rubisco:
Magnesium chelatase and Rubisco were prepared by first freezing a 0.2-g leaf sample in liquid nitrogen to prevent proteolytic activity, followed by grinding with 2 mL of extraction buffer (100 mM phosphate buffer, pH 7.2, containing 0.1 mM EDTA, and 2 mM ascorbic acid). The homogenate was centrifuged for 15 min at 10,000 rpm, and the supernatant was used to measure the protein content (ng L–1), magnesium chelatase activity [pmol(Mg-Deutero) min–1 mg–1(protein)] and Rubisco activity [μmol min–1 mg–1(protein)]. Magnesium chelatase and Rubisco were measured using a magnesium chelatase content kit and a Rubisco content kit (double antibody sandwich method, Huding Biological Technology Co., Shanghai, China) (3 replicates per treatment).
完整版PDF文献请咨询在线客服或者联系我司业务员!
更多公司福利请关注“上海沪鼎”VX公众号!