NEXTFLEX®采用双索引条形码旨在制备多路单端和成对端基因组DNA文库，用于使用Illumina®平台进行测序。索引和流动细胞结合序列包含在NEXTFLEX®Unique Dual index条形码中，并在适配器连接过程中连接到样本插入物上。

Product Overview

The NEXTFLEX®Unique Dual Index Barcodes are designed to prepare multiplexed single and paired-end genomic DNA libraries for sequencing using Illumina® platforms. The index and flow cell binding sequences are contained within the NEXTFLEX® Unique Dual Index Barcodes and attached to the sample insert during adapter ligation. Sample pooling with NEXTFLEX®

Unique Dual Index Barcodes allows the user to multiplex up to 384 samples when used in conjunction with other available sets of NEXTFLEX® Unique Dual Index Barcodes. Uniquely dual-indexed libraries are libraries prepared with adapters containing two eight base indexes: Index 1 (P7 Index) adjacent to the P7 strand, and Index 2 (P5 Index) adjacent to the P5 strand. None of the indexes found on any given NEXTFLEX® Unique Dual Index Barcode are used throughout the entire set, which prevents misassigned reads from appearing in final data sets.

Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing.

Kit Overview

This NEXTFLEX® Unique Dual Index Barcodes Kit contains 8, 24, or 96 uniquely dual- indexed barcoded DNA adapters in tubes or plate format for a total of 16, 48, or 192 reactions.