PlantScreen高通量植物表型成像分析平台（传送带版）（二）

10.根系成像分析

·RhizoTron根窗技术，全自动成像分析，标配根窗44x29.5x5.8cm（高x宽x厚度）

·不仅可对根系成像分析，还可对地上苗（shoot）进行成像分析，苗高*50cm

·新一代CMOS传感器，分辨率12.3MP

·均一LED光源

·3层定位（顶部、中部、底部）根系浇灌系统（选配），3个水箱独立运行

·测量参数包括：根深（或高度）、根冠宽度、高度与宽度比值、根冠面积、根冠紧实度、根系总长、轴对称性、根尖数、根节数等

11.自动浇灌与称重单元

·测量参数：实际重量、浇水体积、*终重量、每个培养盆的相对重量

·操作指令：每个培养盆浇相同量的水（克数或者实际重量的百分比）；保持相对重量；自定义每个培养盆的浇灌量模拟不同干旱或者内涝胁迫；称重前自动零校准，还可通过已知重量（如砝码）物品自动进行再校准

·每个培养盆的浇水量、日期、时间可分别程序控制记录以创建不同干旱胁迫梯度等，并且与整个系统的表型大数据无缝结合分析

·称重精度：大型植物±2g，小型植物±0.2g

·浇灌单元：流速3L/min，浇灌口高度可自动上下前后调整，保证浇灌位置

12.自动化植物传送系统

·传送植物大小：根据客户需求，可达200cm

·传送带容纳量：50盆植物（1000株小型植物），可扩展100盆、200盆、400盆等更大容量 ；表型分析通量依不同的protocol而定，100分钟可以完成整个系统载荷植物样品的表型分析，可随机传送至成像室进行成像分析、随机浇灌

·培养盆：防UV聚丙烯材料，标准5L（口径24cm）培养盆，可通过适配器应用3L培养盆，可360度旋转

·具备手动载样环（manual loading loop）以便在系统待机模式下手动载样分析实验、小组实验分析等

·具备激光植物高度测量监测系统和*

·环形传送通道：具变速箱的三相异步马达，功率200-1000W，*负载500kg，速度150mm/s，传送带材料为防UV高耐用PVC

·移动控制系统：*处理单元CJ2M-CPU33；数字输入/输出*2560点；输入/输出单元*40；温度传感器Pt1000，Pt100，PTC；PLC通讯百兆以太网；OMRON MECHATROLINK-II *16轴精确定位

·RFID标签和QR植物辨识系统，自动读取每个样品托盘上的二维编码；辨识距离2-20cm；通讯RS485；可读取1维、2维和QR码；配备LED光源便于弱光下辨识

·环境监测传感器：温湿度传感器、PAR光合有效辐射传感器

·由主控制系统分别自动调控每一个样品托盘的测量时间、测量顺序、测量参数、浇灌时间和浇灌量，从测量单元到培养室的样品运转整个过程可实现*自动控制，在无人值守情况下根据预设程序自行完成全部实验测量工作。

13.主控制表型大数据平台

·组成：控制调度服务器、客户端应用服务器、数据服务器、可编程序逻辑控制器及专业分析软件等，数据容量12TB

·自动控制与分析功能：具备用户定义、可编辑自动测量程序（protocols），根据用户设定程序自动完成全部实验。数据结果自动存储并分析，分析的数据结果可自动以动态曲线的形式显示。

·MySQL数据库管理系统，可以处理拥有上千万条记录的大型数据库，支持多种存储引擎，相关数据自动存储于数据库中的不同表中

·植物编码注册功能：包括植物识别码、所在托盘的识别码等存储在数据库中，测量时自动提取自动读取条形码或RFID标签

·触摸屏操作界面，在线显示植物托盘数量、光线强度、分析测量状态及结果等，轻松通过软件*控制所有的机械部件和成像工作站

·可用默认程序进行所有测量，也可通过开发工具创建自定义的工作过程，或者手动操作LED光源开启或关闭、RGB成像、叶绿素荧光成像、高光谱成像、红外热成像、3D激光扫描、称重及浇灌等

·叶片跟踪监测功能（leaf tracking）模块，可以持续跟踪监测叶片的生长、变化等等

·3D投射技术，可以通过高分辨率RGB镜头 或激光扫描构建3D模型，通过投射技术，将与其它传感器所得数据如叶绿素荧光、红外热成像温度数据、近红外数据、高光谱数据等投射在3D模型上一起进行对比分析等

·允许用户通过互联网远程访问，进行数据处理、下载及更改实验设计

·所测量的所有数据都是透明的、可以追溯的

·具备用户权限分级功能，防止其他人员误操作影响实验

·厂家远程故障诊断，软件*升级

执行标准：

·CE认证标准

·CSN EN 60529 防护等级标准

·CSN 33 01 65 导体侧识别标准

·CSN 33 2000-3 基础特性标准

·CSN 33 2000-4-41ed.2 电击保护标准

·CSN 33 2000-4-43 电源过载保护标准

·CSN 33 2000-5-51ed.2 通用规则标准

·CSN 33 2000-5-523 容许电流标准

·CSN 33 2000-5-54ed.2 接地与保护导体标准

·CSN EN 55011 工业、科学与医学设备测量电磁干扰的范围与方法

·2006/42/EG 机械指令标准

·73/23/EEG 低电压指令标准

·2004/108/EG 电磁相容性指令标准

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附：其它表型分析平台：

1、FKM多光谱荧光动态显微成像系统

右图引自《Nature Plants》2016, Photonic multilayer structure of Begonia chloroplasts enhances photosynthetic efficiency by Heather M. Whitney等

2、PlantScreen-R移动式表型分析平台（下左图）：用于大田植物叶绿素荧光成像分析、RGB成像分析、红外热成像分析、3D激光扫描测量分析等

3、PlantScreen台式及移动式植物表型分析平台（参见上右图）

1)3D RGB彩色成像分析

2)FluorCam叶绿素荧光成像分析

3)FluorCam多光谱荧光成像分析

4)高光谱成像分析

5)红外热成像分析

6)PAR吸收／NDVI成像分析

7)近红外3D成像分析

4、PlantScreen样带式表型分析平台

5、PlantScreen 植物表型三维自动扫描成像分析平台