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叶绿素荧光系统成像仪——PAM-WATCH

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2023-08-03上海
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叶绿素荧光系统成像仪——PAM-WATCH
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功能大、操作、发表文献最多的叶绿素荧光成像系统

突变株快速筛选的强大工具


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•  标准蓝光版,450 nm,测叶片和真核藻类

•  红光版,620 nm,测蓝藻和真核藻类

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主要功能

•  成像功能:对Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等参数进行成像分析。测定调节性能量耗散Y(NPQ),反映植物光保护能力,测定非调节性能量耗散Y(NO),反映植物光损伤程度。

•  程序测量功能:可程序测量荧光诱导曲线、快速光曲线和暗弛豫,也可手动测量;在测量过程中能自动分析所有荧光参数的变化趋势

•  AOI功能:可在测量前或测量后任意选择感兴趣的区域(AOI),程序将自动对选择的AOI的数据进行变化趋势分析,并在报告文件中显示相关AOI的数据。所有报告文件中显示的数据都可导出到EXCEL文件中。

•  成像异质性分析功能:对任意参数任意时间的成像,可在图像上任意选取两点,软件自动对两点间的数据进行横向异质性分析,并可导出到EXCEL文件中。

•  成像数据范围分析功能:对任意参数任意时间的成像,可分析任意两个荧光数值之间有多少个像素点,多少面积(cm2)。

•  突变株筛选功能:可跟据成像结果快速筛选光合、产氢/油、抗逆(抗盐、抗旱、抗病等)等突变株

•  微藻毒理研究功能:可同时测量96个微藻样品(对照和处理组)的光合活性,软件自动给出处理组样品相对于对照组的光合抑制百分比。

•  吸光系数测量功能:快速测量叶片的吸光系数。吸光系数测量光源: 16个红光(650 nm)和16个近红外(780 nm)LED,用于测量植物叶片或藻类样品PAR吸光系数。

•  监测功能:可实时监控箱内环境状态、机器状态监控区显示目前程序及环境状态

•  环境仿真功能:可程序化多组程序功能 (最多6组),可个别设定执行天数,可设备单日环境参数(最小间隔为分),程序化环境参数

•  项目管理功能:预约存多次测量的植物影像及参数,延时(缩时)摄影功能

•  数据分析功能:可导出照片文件,可导出叶绿素荧光参数Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等


系统设计

•  可单独作为叶绿素荧光仪使用,方便带到野外田间测量和成像;也可配置箱体式结构,容量300L,能同时满足植物培养和叶绿素荧光成像的功能。箱体内设有栽培区,可分2层、3层、4层等,调节高度进行培养和测量。箱体内有特殊消光图层,避免反光造成的测量干扰。

•  系统配置触摸式高清显示屏(24寸),方便操作,支持1670万色彩和FHD高清画质。

•  系统处理器:I7处理器,16G缓存,512固态硬盘SSD,满足快速数据处理和存储要求。

•  供电:室内使用220V,50Hz交流电,荧光仪单独使用时,内置可充电锂电池供电,满足4小时的野外测量


箱体环境控制(光照、温度等)

•  温度环控条件:15-40°C ±5°C(关灯)

•  光照系统:采用四合一LED光盘,白光5000k ±500k、红光660nm±10nm、蓝450nm±10nm、红外光730nm±10nm,光盘面积29×40cm,四种光源可独立自主控制,光质强度PPFD ≧700µmols-1m-2@15cm,光照时间控制可程序化设定,最小设定时间1min,可仿真日出至日落光照变化。

•  控制系统:PID微电脑控制,可设定上昼夜循环程序

•  可选配二氧化碳和湿度控制单元


成像技术参数

Maxi探头

•  荧光测量光源: 44个蓝色LED,450 nm,测量光强度0.5 μmol m-2 s-1PAR,光化光强度1900 μmol m-2 s-1PAR,饱和脉冲强度4000 μmol m-2 s-1PAR

•  吸光系数测量光源:16个红光(660 nm)和16个近红外(780 nm)LED,用于测量样品PAR吸光系数。

•  成像面积:工作距离18.5cm,成像面积 10×13 cm;工作距离22.5cm,成像面积11×15cm。

•  光强异质性:测量区域光强异质性小于±7%。

•  测量参数:Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等。


应用领域

•  光合作用研究:可以在相同的条件下同时对大量样品进行成像

•  植物病理学:病斑部位(包括肉眼不可见时)成像以及病斑扩散的时空动力学

•  植物胁迫生 理学:肉眼不可见胁迫损伤的早期检测

•  遗传育种:出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株

•  突变株筛选:快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等

•  微藻毒理学:不同毒物浓度多个重复的样品一次测完,软件自动计算抑制比率

•  分子生物学:宏观水平上检测样品的绿色荧光蛋白(GFP)荧光

•  其它多种扩展研究


成像参数

 Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等

 

突变株的快速筛选

MAXI-IMAGING-PAM特别适合对幼苗、愈伤组织、微藻等进行突变株的快速筛选,适合于与光合突变株、抗逆(抗旱、抗盐、抗病等)突变株、产油/氢突变株等的快速筛选。

 

国外利用MAXI-IMAGING-PAM筛选突变株的典型客户如拜耳、BASF、孟山都、先正达等大型跨国农业,以及各大农业育种、植物分子生物学等科研单位,例如澳大利亚植物功能基因组中心(阿德雷德大学)、德国尤里希表型植物表型研究中心(Julich Plant Phenotyping Centre)等等。

 

国内约一半的MAXI-IMAGING-PAM客户在进行突变株快速筛选工作,主要分布于、中国农科院和各大高校。


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突变株筛选实例一:国内某客户筛选的拟南芥突变株

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突变株筛选实例二:产油突变株的筛选。Ajjawi et al, 2010, Plant Physiol., 152: 529-540.

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突变株筛选实例三:光合突变株的筛选。Armbruster et al., 2010, Plant Cell, 22: 3439-3460.


调制叶绿素荧光成像实例

叶片成像异质性

1)葡萄叶片

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2)荷花叶片

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水果的成像

1)草莓的成像

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2)猕猴桃的成像

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突变株筛选

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植物病理研究

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荧光成像与CO2气体交换的同步测量

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(GFS-3000/IM-MAXI):MAXI-探头与GFS-3000联用,在10 cm x 13 cm的面积上同步测量气体交换与荧光成像。


产地:德国WALZ

 

代表文献

数据来源:光合作用文献Endnote数据库

原始数据来源:Google Scholar

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